The cytoplasmic domain of Sso1 and truncation constructs were dialyzed against 20 mM sodium phosphate buffer, pH 6.5, containing 50 mM NaCl. Protein concentrations were determined by internally ...
Mix one volume of this 2× calcium phosphate–DNA solution with an equal volume of 2× HEPES-buffered saline (HBS) at 15–25 °C. Tap the side of the tube to mix and incubate the solution at 15 ...
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