Nenana (Lower Tanana: Toghotili; is a home rule city in the Yukon-Koyukuk Census Area of the Unorganized Borough in Interior Alaska…Nenana (Lower Tanana: Toghotili; is a home rule city in the Yukon-Koyukuk Census Area of the Unorganized Borough in Interior Alaska. Nenana developed as a Lower Tanana community at the confluence where the tributary Nenana River enters the Tanana. The population was 378 at the 2010 census, down from 402 in 2000.